Source of the materials: Biopython cookbook (adapted) Status: Draft

BLAST and other sequence search tools (experimental code)

WARNING: This chapter of the Tutorial describes an experimental module in Biopython. It is being included in Biopython and documented here in the tutorial in a pre-final state to allow a period of feedback and refinement before we declare it stable. Until then the details will probably change, and any scripts using the current Bio.SearchIO would need to be updated. Please keep this in mind! For stable code working with NCBI BLAST, please continue to use Bio.Blast described in the preceding Chapter [chapter:blast].

Biological sequence identification is an integral part of bioinformatics. Several tools are available for this, each with their own algorithms and approaches, such as BLAST (arguably the most popular), FASTA, HMMER, and many more. In general, these tools usually use your sequence to search a database of potential matches. With the growing number of known sequences (hence the growing number of potential matches), interpreting the results becomes increasingly hard as there could be hundreds or even thousands of potential matches. Naturally, manual interpretation of these searches’ results is out of the question. Moreover, you often need to work with several sequence search tools, each with its own statistics, conventions, and output format. Imagine how daunting it would be when you need to work with multiple sequences using multiple search tools.

We know this too well ourselves, which is why we created the Bio.SearchIO submodule in Biopython. Bio.SearchIO allows you to extract information from your search results in a convenient way, while also dealing with the different standards and conventions used by different search tools. The name SearchIO is a homage to BioPerl’s module of the same name.

In this chapter, we’ll go through the main features of Bio.SearchIO to show what it can do for you. We’ll use two popular search tools along the way: BLAST and BLAT. They are used merely for illustrative purposes, and you should be able to adapt the workflow to any other search tools supported by Bio.SearchIO in a breeze. You’re very welcome to follow along with the search output files we’ll be using. The BLAST output file can be downloaded here, and the BLAT output file here. Both output files were generated using this sequence:

>mystery_seq
CCCTCTACAGGGAAGCGCTTTCTGTTGTCTGAAAGAAAAGAAAGTGCTTCCTTTTAGAGGG

The BLAST result is an XML file generated using blastn against the NCBI refseq_rna database. For BLAT, the sequence database was the February 2009 hg19 human genome draft and the output format is PSL.

We’ll start from an introduction to the Bio.SearchIO object model. The model is the representation of your search results, thus it is core to Bio.SearchIO itself. After that, we’ll check out the main functions in Bio.SearchIO that you may often use.

Now that we’re all set, let’s go to the first step: introducing the core object model.

The SearchIO object model

Despite the wildly differing output styles among many sequence search tools, it turns out that their underlying concept is similar:

• The output file may contain results from one or more search queries.
• In each search query, you will see one or more hits from the given search database.
• In each database hit, you will see one or more regions containing the actual sequence alignment between your query sequence and the database sequence.
• Some programs like BLAT or Exonerate may further split these regions into several alignment fragments (or blocks in BLAT and possibly exons in exonerate). This is not something you always see, as programs like BLAST and HMMER do not do this.

Realizing this generality, we decided use it as base for creating the Bio.SearchIO object model. The object model consists of a nested hierarchy of Python objects, each one representing one concept outlined above. These objects are:

• QueryResult, to represent a single search query.
• Hit, to represent a single database hit. Hit objects are contained within QueryResult and in each QueryResult there is zero or more Hit objects.
• HSP (short for high-scoring pair), to represent region(s) of significant alignments between query and hit sequences. HSP objects are contained within Hit objects and each Hit has one or more HSP objects.
• HSPFragment, to represent a single contiguous alignment between query and hit sequences. HSPFragment objects are contained within HSP objects. Most sequence search tools like BLAST and HMMER unify HSP and HSPFragment objects as each HSP will only have a single HSPFragment. However there are tools like BLAT and Exonerate that produce HSP containing multiple HSPFragment. Don’t worry if this seems a tad confusing now, we’ll elaborate more on these two objects later on.

These four objects are the ones you will interact with when you use Bio.SearchIO. They are created using one of the main Bio.SearchIO methods: read, parse, index, or index_db. The details of these methods are provided in later sections. For this section, we’ll only be using read and parse. These functions behave similarly to their Bio.SeqIO and Bio.AlignIO counterparts:

• read is used for search output files with a single query and returns a QueryResult object
• parse is used for search output files with multiple queries and returns a generator that yields QueryResult objects

With that settled, let’s start probing each Bio.SearchIO object, beginning with QueryResult.

QueryResult

The QueryResult object represents a single search query and contains zero or more Hit objects. Let’s see what it looks like using the BLAST file we have:

from Bio import SearchIO
blast_qresult = SearchIO.read('data/my_blast.xml', 'blast-xml')
print(blast_qresult)

Program: blastn (2.3.0+)
  Query: gi|8332116|gb|BE037100.1|BE037100 (1111)
         MP14H09 MP Mesembryanthemum crystallinum cDNA 5' similar to cold ac...
 Target: nt
   Hits: ----  -----  ----------------------------------------------------------
            #  # HSP  ID + description
         ----  -----  ----------------------------------------------------------
            0      1  gi|731339628|ref|XM_010682658.1|  PREDICTED: Beta vulga...
            1      1  gi|568824607|ref|XM_006466626.1|  PREDICTED: Citrus sin...
            2      1  gi|568824605|ref|XM_006466625.1|  PREDICTED: Citrus sin...
            3      1  gi|568824603|ref|XM_006466624.1|  PREDICTED: Citrus sin...
            4      1  gi|568824601|ref|XM_006466623.1|  PREDICTED: Citrus sin...
            5      1  gi|568824599|ref|XM_006466622.1|  PREDICTED: Citrus sin...
            6      1  gi|567866318|ref|XM_006425719.1|  Citrus clementina hyp...
            7      1  gi|567866316|ref|XM_006425718.1|  Citrus clementina hyp...
            8      1  gi|567866314|ref|XM_006425717.1|  Citrus clementina hyp...
            9      1  gi|567866312|ref|XM_006425716.1|  Citrus clementina hyp...
           10      1  gi|590704208|ref|XM_007047033.1|  Theobroma cacao Cold-...
           11      1  gi|590704205|ref|XM_007047032.1|  Theobroma cacao Cold-...
           12      1  gi|694428700|ref|XM_009343631.1|  PREDICTED: Pyrus x br...
           13      1  gi|694402986|ref|XM_009378191.1|  PREDICTED: Pyrus x br...
           14      1  gi|743838297|ref|XM_011027373.1|  PREDICTED: Populus eu...
           15      1  gi|743838293|ref|XM_011027372.1|  PREDICTED: Populus eu...
           16      1  gi|595807351|ref|XM_007202530.1|  Prunus persica hypoth...
           17      1  gi|566180892|ref|XM_006380679.1|  Populus trichocarpa c...
           18      1  gi|764593175|ref|XM_004300526.2|  PREDICTED: Fragaria v...
           19      1  gi|731440276|ref|XM_002274845.3|  PREDICTED: Vitis vini...
           20      1  gi|349709091|emb|FQ378501.1|  Vitis vinifera clone SS0A...
           21      1  gi|719997221|ref|XM_010256725.1|  PREDICTED: Nelumbo nu...
           22      1  gi|645272858|ref|XM_008243375.1|  PREDICTED: Prunus mum...
           23      1  gi|645272856|ref|XM_008243374.1|  PREDICTED: Prunus mum...
           24      1  gi|848856318|ref|XM_013000712.1|  PREDICTED: Erythranth...
           25      1  gi|255562758|ref|XM_002522339.1|  Ricinus communis COR4...
           26      1  gi|658006068|ref|XM_008339966.1|  PREDICTED: Malus x do...
           27      1  gi|802641059|ref|XM_012223735.1|  PREDICTED: Jatropha c...
           28      1  gi|802641054|ref|XM_012223734.1|  PREDICTED: Jatropha c...
           29      1  gi|823184330|ref|XM_012633708.1|  PREDICTED: Gossypium ...
           ~~~
           47      1  gi|729314350|ref|XM_010532905.1|  PREDICTED: Tarenaya h...
           48      1  gi|731383573|ref|XM_002284686.2|  PREDICTED: Vitis vini...
           49      1  gi|255762732|gb|GQ370517.1|  Salvia miltiorrhiza cold a...

/home/tiago_antao/miniconda/lib/python3.5/site-packages/Bio/SearchIO/__init__.py:211: BiopythonExperimentalWarning: Bio.SearchIO is an experimental submodule which may undergo significant changes prior to its future official release.
  BiopythonExperimentalWarning)

We’ve just begun to scratch the surface of the object model, but you can see that there’s already some useful information. By invoking print on the QueryResult object, you can see:

• The program name and version (blastn version 2.2.27+)
• The query ID, description, and its sequence length (ID is 42291, description is ‘mystery_seq’, and it is 61 nucleotides long)
• The target database to search against (refseq_rna)
• A quick overview of the resulting hits. For our query sequence, there are 100 potential hits (numbered 0–99 in the table). For each hit, we can also see how many HSPs it contains, its ID, and a snippet of its description. Notice here that Bio.SearchIO truncates the hit table overview, by showing only hits numbered 0–29, and then 97–99.

Now let’s check our BLAT results using the same procedure as above:

blat_qresult = SearchIO.read('data/my_blat.psl', 'blat-psl')
print(blat_qresult)

Program: blat (<unknown version>)
  Query: mystery_seq (61)
         <unknown description>
 Target: <unknown target>
   Hits: ----  -----  ----------------------------------------------------------
            #  # HSP  ID + description
         ----  -----  ----------------------------------------------------------
            0     17  chr19  <unknown description>

You’ll immediately notice that there are some differences. Some of these are caused by the way PSL format stores its details, as you’ll see. The rest are caused by the genuine program and target database differences between our BLAST and BLAT searches:

• The program name and version. Bio.SearchIO knows that the program is BLAT, but in the output file there is no information regarding the program version so it defaults to ‘<unknown version>’.
• The query ID, description, and its sequence length. Notice here that these details are slightly different from the ones we saw in BLAST. The ID is ‘mystery_seq’ instead of 42991, there is no known description, but the query length is still 61. This is actually a difference introduced by the file formats themselves. BLAST sometimes creates its own query IDs and uses your original ID as the sequence description.
• The target database is not known, as it is not stated in the BLAT output file.
• And finally, the list of hits we have is completely different. Here, we see that our query sequence only hits the ‘chr19’ database entry, but in it we see 17 HSP regions. This should not be surprising however, given that we are using a different program, each with its own target database.

All the details you saw when invoking the print method can be accessed individually using Python’s object attribute access notation (a.k.a. the dot notation). There are also other format-specific attributes that you can access using the same method.

print("%s %s" % (blast_qresult.program, blast_qresult.version))

blastn 2.3.0+

print("%s %s" % (blat_qresult.program, blat_qresult.version))

blat <unknown version>

blast_qresult.param_evalue_threshold    # blast-xml specific

10.0

For a complete list of accessible attributes, you can check each format-specific documentation. Here are the ones for BLAST and for BLAT.

Having looked at using print on QueryResult objects, let’s drill down deeper. What exactly is a QueryResult? In terms of Python objects, QueryResult is a hybrid between a list and a dictionary. In other words, it is a container object with all the convenient features of lists and dictionaries.

Like Python lists and dictionaries, QueryResult objects are iterable. Each iteration returns a Hit object:

for hit in blast_qresult:
    hit

To check how many items (hits) a QueryResult has, you can simply invoke Python’s len method:

len(blast_qresult)

50

len(blat_qresult)

1

Like Python lists, you can retrieve items (hits) from a QueryResult using the slice notation:

blast_qresult[0]        # retrieves the top hit

Hit(id='gi|731339628|ref|XM_010682658.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps)

blast_qresult[-1]       # retrieves the last hit

Hit(id='gi|255762732|gb|GQ370517.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps)

To retrieve multiple hits, you can slice QueryResult objects using the slice notation as well. In this case, the slice will return a new QueryResult object containing only the sliced hits:

blast_slice = blast_qresult[:3]     # slices the first three hits
print(blast_slice)

Program: blastn (2.3.0+)
  Query: gi|8332116|gb|BE037100.1|BE037100 (1111)
         MP14H09 MP Mesembryanthemum crystallinum cDNA 5' similar to cold ac...
 Target: nt
   Hits: ----  -----  ----------------------------------------------------------
            #  # HSP  ID + description
         ----  -----  ----------------------------------------------------------
            0      1  gi|731339628|ref|XM_010682658.1|  PREDICTED: Beta vulga...
            1      1  gi|568824607|ref|XM_006466626.1|  PREDICTED: Citrus sin...
            2      1  gi|568824605|ref|XM_006466625.1|  PREDICTED: Citrus sin...

Like Python dictionaries, you can also retrieve hits using the hit’s ID. This is particularly useful if you know a given hit ID exists within a search query results:

blast_qresult['gi|731339628|ref|XM_010682658.1|']

Hit(id='gi|731339628|ref|XM_010682658.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps)

You can also get a full list of Hit objects using hits and a full list of Hit IDs using hit_keys:

blast_qresult.hits

[Hit(id='gi|731339628|ref|XM_010682658.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|568824607|ref|XM_006466626.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|568824605|ref|XM_006466625.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|568824603|ref|XM_006466624.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|568824601|ref|XM_006466623.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|568824599|ref|XM_006466622.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|567866318|ref|XM_006425719.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|567866316|ref|XM_006425718.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|567866314|ref|XM_006425717.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|567866312|ref|XM_006425716.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|590704208|ref|XM_007047033.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|590704205|ref|XM_007047032.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|694428700|ref|XM_009343631.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|694402986|ref|XM_009378191.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|743838297|ref|XM_011027373.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|743838293|ref|XM_011027372.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|595807351|ref|XM_007202530.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|566180892|ref|XM_006380679.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|764593175|ref|XM_004300526.2|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|731440276|ref|XM_002274845.3|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|349709091|emb|FQ378501.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|719997221|ref|XM_010256725.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|645272858|ref|XM_008243375.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|645272856|ref|XM_008243374.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|848856318|ref|XM_013000712.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|255562758|ref|XM_002522339.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|658006068|ref|XM_008339966.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|802641059|ref|XM_012223735.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|802641054|ref|XM_012223734.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|823184330|ref|XM_012633708.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|697116717|ref|XM_009613983.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|698554552|ref|XM_009771948.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|565355903|ref|XM_006344753.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|659079297|ref|XM_008441961.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|659079295|ref|XM_008441960.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|828326432|ref|XM_004509143.2|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|729300104|ref|XM_010559563.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|922336892|ref|XM_013590982.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|955375440|ref|XM_003548859.3|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|778718146|ref|XM_011659511.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|778718144|ref|XM_004141977.2|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|955306249|ref|XM_003519866.3|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|747102191|ref|XM_011100949.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|723677217|ref|XM_004233368.2|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|703126872|ref|XM_010105390.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|658037046|ref|XM_008355863.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|225311746|dbj|AK326681.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|729314350|ref|XM_010532905.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|731383573|ref|XM_002284686.2|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps),
 Hit(id='gi|255762732|gb|GQ370517.1|', query_id='gi|8332116|gb|BE037100.1|BE037100', 1 hsps)]

blast_qresult.hit_keys

['gi|731339628|ref|XM_010682658.1|',
 'gi|568824607|ref|XM_006466626.1|',
 'gi|568824605|ref|XM_006466625.1|',
 'gi|568824603|ref|XM_006466624.1|',
 'gi|568824601|ref|XM_006466623.1|',
 'gi|568824599|ref|XM_006466622.1|',
 'gi|567866318|ref|XM_006425719.1|',
 'gi|567866316|ref|XM_006425718.1|',
 'gi|567866314|ref|XM_006425717.1|',
 'gi|567866312|ref|XM_006425716.1|',
 'gi|590704208|ref|XM_007047033.1|',
 'gi|590704205|ref|XM_007047032.1|',
 'gi|694428700|ref|XM_009343631.1|',
 'gi|694402986|ref|XM_009378191.1|',
 'gi|743838297|ref|XM_011027373.1|',
 'gi|743838293|ref|XM_011027372.1|',
 'gi|595807351|ref|XM_007202530.1|',
 'gi|566180892|ref|XM_006380679.1|',
 'gi|764593175|ref|XM_004300526.2|',
 'gi|731440276|ref|XM_002274845.3|',
 'gi|349709091|emb|FQ378501.1|',
 'gi|719997221|ref|XM_010256725.1|',
 'gi|645272858|ref|XM_008243375.1|',
 'gi|645272856|ref|XM_008243374.1|',
 'gi|848856318|ref|XM_013000712.1|',
 'gi|255562758|ref|XM_002522339.1|',
 'gi|658006068|ref|XM_008339966.1|',
 'gi|802641059|ref|XM_012223735.1|',
 'gi|802641054|ref|XM_012223734.1|',
 'gi|823184330|ref|XM_012633708.1|',
 'gi|697116717|ref|XM_009613983.1|',
 'gi|698554552|ref|XM_009771948.1|',
 'gi|565355903|ref|XM_006344753.1|',
 'gi|659079297|ref|XM_008441961.1|',
 'gi|659079295|ref|XM_008441960.1|',
 'gi|828326432|ref|XM_004509143.2|',
 'gi|729300104|ref|XM_010559563.1|',
 'gi|922336892|ref|XM_013590982.1|',
 'gi|955375440|ref|XM_003548859.3|',
 'gi|778718146|ref|XM_011659511.1|',
 'gi|778718144|ref|XM_004141977.2|',
 'gi|955306249|ref|XM_003519866.3|',
 'gi|747102191|ref|XM_011100949.1|',
 'gi|723677217|ref|XM_004233368.2|',
 'gi|703126872|ref|XM_010105390.1|',
 'gi|658037046|ref|XM_008355863.1|',
 'gi|225311746|dbj|AK326681.1|',
 'gi|729314350|ref|XM_010532905.1|',
 'gi|731383573|ref|XM_002284686.2|',
 'gi|255762732|gb|GQ370517.1|']

What if you just want to check whether a particular hit is present in the query results? You can do a simple Python membership test using the in keyword:

'gi|262205317|ref|NR_030195.1|' in blast_qresult

False

'gi|731339628|ref|XM_010682658.1|' in blast_qresult

True

Sometimes, knowing whether a hit is present is not enough; you also want to know the rank of the hit. Here, the index method comes to the rescue:

blast_qresult.index('gi|595807351|ref|XM_007202530.1|')

16

Remember that we’re using Python’s indexing style here, which is zero-based. This means our hit above is ranked at no. 23, not 22.

Also, note that the hit rank you see here is based on the native hit ordering present in the original search output file. Different search tools may order these hits based on different criteria.

If the native hit ordering doesn’t suit your taste, you can use the sort method of the QueryResult object. It is very similar to Python’s list.sort method, with the addition of an option to create a new sorted QueryResult object or not.

Here is an example of using QueryResult.sort to sort the hits based on each hit’s full sequence length. For this particular sort, we’ll set the in_place flag to False so that sorting will return a new QueryResult object and leave our initial object unsorted. We’ll also set the reverse flag to True so that we sort in descending order.

for hit in blast_qresult[:5]:   # id and sequence length of the first five hits
    print("%s %i" % (hit.id, hit.seq_len))

gi|731339628|ref|XM_010682658.1| 847
gi|568824607|ref|XM_006466626.1| 878
gi|568824605|ref|XM_006466625.1| 911
gi|568824603|ref|XM_006466624.1| 894
gi|568824601|ref|XM_006466623.1| 922

sort_key = lambda hit: hit.seq_len
sorted_qresult = blast_qresult.sort(key=sort_key, reverse=True, in_place=False)
for hit in sorted_qresult[:5]:
    print("%s %i" % (hit.id, hit.seq_len))

gi|955306249|ref|XM_003519866.3| 1388
gi|659079295|ref|XM_008441960.1| 1245
gi|567866316|ref|XM_006425718.1| 1202
gi|566180892|ref|XM_006380679.1| 1182
gi|565355903|ref|XM_006344753.1| 1153

The advantage of having the in_place flag here is that we’re preserving the native ordering, so we may use it again later. You should note that this is not the default behavior of QueryResult.sort, however, which is why we needed to set the in_place flag to True explicitly.

At this point, you’ve known enough about QueryResult objects to make it work for you. But before we go on to the next object in the Bio.SearchIO model, let’s take a look at two more sets of methods that could make it even easier to work with QueryResult objects: the filter and map methods.

If you’re familiar with Python’s list comprehensions, generator expressions or the built in filter and map functions, you’ll know how useful they are for working with list-like objects (if you’re not, check them out!). You can use these built in methods to manipulate QueryResult objects, but you’ll end up with regular Python lists and lose the ability to do more interesting manipulations.

That’s why, QueryResult objects provide its own flavor of filter and map methods. Analogous to filter, there are hit_filter and hsp_filter methods. As their name implies, these methods filter its QueryResult object either on its Hit objects or HSP objects. Similarly, analogous to map, QueryResult objects also provide the hit_map and hsp_map methods. These methods apply a given function to all hits or HSPs in a QueryResult object, respectively.

Let’s see these methods in action, beginning with hit_filter. This method accepts a callback function that checks whether a given Hit object passes the condition you set or not. In other words, the function must accept as its argument a single Hit object and returns True or False.

Here is an example of using hit_filter to filter out Hit objects that only have one HSP:

filter_func = lambda hit: len(hit.hsps) > 1     # the callback function
len(blast_qresult)      # no. of hits before filtering

50

filtered_qresult = blast_qresult.hit_filter(filter_func)
len(filtered_qresult)   # no. of hits after filtering

0

for hit in filtered_qresult[:5]:    # quick check for the hit lengths
    print("%s %i" % (hit.id, len(hit.hsps)))

hsp_filter works the same as hit_filter, only instead of looking at the Hit objects, it performs filtering on the HSP objects in each hits.

As for the map methods, they too accept a callback function as their arguments. However, instead of returning True or False, the callback function must return the modified Hit or HSP object (depending on whether you’re using hit_map or hsp_map).

Let’s see an example where we’re using hit_map to rename the hit IDs:

def map_func(hit):
    hit.id = hit.id.split('|')[3]   # renames 'gi|301171322|ref|NR_035857.1|' to 'NR_035857.1'
    return hit

mapped_qresult = blast_qresult.hit_map(map_func)
for hit in mapped_qresult[:5]:
    print(hit.id)

XM_010682658.1
XM_006466626.1
XM_006466625.1
XM_006466624.1
XM_006466623.1

Again, hsp_map works the same as hit_map, but on HSP objects instead of Hit objects.

Hit

Hit objects represent all query results from a single database entry. They are the second-level container in the Bio.SearchIO object hierarchy. You’ve seen that they are contained by QueryResult objects, but they themselves contain HSP objects.

Let’s see what they look like, beginning with our BLAST search:

from Bio import SearchIO
blast_qresult = SearchIO.read('data/my_blast.xml', 'blast-xml')
blast_hit = blast_qresult[3]    # fourth hit from the query result
print(blast_hit)

Query: gi|8332116|gb|BE037100.1|BE037100
       MP14H09 MP Mesembryanthemum crystallinum cDNA 5' similar to cold accl...
  Hit: gi|568824603|ref|XM_006466624.1| (894)
       PREDICTED: Citrus sinensis cold-regulated 413 plasma membrane protein...
 HSPs: ----  --------  ---------  ------  ---------------  ---------------------
          #   E-value  Bit score    Span      Query range              Hit range
       ----  --------  ---------  ------  ---------------  ---------------------
          0   6.9e-99     372.78     596         [63:655]              [108:697]

You see that we’ve got the essentials covered here:

• The query ID and description is present. A hit is always tied to a query, so we want to keep track of the originating query as well. These values can be accessed from a hit using the query_id and query_description attributes.
• We also have the unique hit ID, description, and full sequence lengths. They can be accessed using id, description, and seq_len, respectively.
• Finally, there’s a table containing quick information about the HSPs this hit contains. In each row, we’ve got the important HSP details listed: the HSP index, its e-value, its bit score, its span (the alignment length including gaps), its query coordinates, and its hit coordinates.

Now let’s contrast this with the BLAT search. Remember that in the BLAT search we had one hit with 17 HSPs.

blat_qresult = SearchIO.read('data/my_blat.psl', 'blat-psl')
blat_hit = blat_qresult[0]      # the only hit
print(blat_hit)

Query: mystery_seq
       <unknown description>
  Hit: chr19 (59128983)
       <unknown description>
 HSPs: ----  --------  ---------  ------  ---------------  ---------------------
          #   E-value  Bit score    Span      Query range              Hit range
       ----  --------  ---------  ------  ---------------  ---------------------
          0         ?          ?       ?           [0:61]    [54204480:54204541]
          1         ?          ?       ?           [0:61]    [54233104:54264463]
          2         ?          ?       ?           [0:61]    [54254477:54260071]
          3         ?          ?       ?           [1:61]    [54210720:54210780]
          4         ?          ?       ?           [0:60]    [54198476:54198536]
          5         ?          ?       ?           [0:61]    [54265610:54265671]
          6         ?          ?       ?           [0:61]    [54238143:54240175]
          7         ?          ?       ?           [0:60]    [54189735:54189795]
          8         ?          ?       ?           [0:61]    [54185425:54185486]
          9         ?          ?       ?           [0:60]    [54197657:54197717]
         10         ?          ?       ?           [0:61]    [54255662:54255723]
         11         ?          ?       ?           [0:61]    [54201651:54201712]
         12         ?          ?       ?           [8:60]    [54206009:54206061]
         13         ?          ?       ?          [10:61]    [54178987:54179038]
         14         ?          ?       ?           [8:61]    [54212018:54212071]
         15         ?          ?       ?           [8:51]    [54234278:54234321]
         16         ?          ?       ?           [8:61]    [54238143:54238196]

Here, we’ve got a similar level of detail as with the BLAST hit we saw earlier. There are some differences worth explaining, though:

• The e-value and bit score column values. As BLAT HSPs do not have e-values and bit scores, the display defaults to ‘?’.
• What about the span column? The span values is meant to display the complete alignment length, which consists of all residues and any gaps that may be present. The PSL format do not have this information readily available and Bio.SearchIO does not attempt to try guess what it is, so we get a ‘?’ similar to the e-value and bit score columns.

In terms of Python objects, Hit behaves almost the same as Python lists, but contain HSP objects exclusively. If you’re familiar with lists, you should encounter no difficulties working with the Hit object.

Just like Python lists, Hit objects are iterable, and each iteration returns one HSP object it contains:

for hsp in blast_hit:
    hsp

You can invoke len on a Hit to see how many HSP objects it has:

len(blast_hit)

1

len(blat_hit)

17

You can use the slice notation on Hit objects, whether to retrieve single HSP or multiple HSP objects. Like QueryResult, if you slice for multiple HSP, a new Hit object will be returned containing only the sliced HSP objects:

blat_hit[0]                 # retrieve single items

HSP(hit_id='chr19', query_id='mystery_seq', 1 fragments)

sliced_hit = blat_hit[4:9]  # retrieve multiple items
len(sliced_hit)

5

print(sliced_hit)

Query: mystery_seq
       <unknown description>
  Hit: chr19 (59128983)
       <unknown description>
 HSPs: ----  --------  ---------  ------  ---------------  ---------------------
          #   E-value  Bit score    Span      Query range              Hit range
       ----  --------  ---------  ------  ---------------  ---------------------
          0         ?          ?       ?           [0:60]    [54198476:54198536]
          1         ?          ?       ?           [0:61]    [54265610:54265671]
          2         ?          ?       ?           [0:61]    [54238143:54240175]
          3         ?          ?       ?           [0:60]    [54189735:54189795]
          4         ?          ?       ?           [0:61]    [54185425:54185486]

You can also sort the HSP inside a Hit, using the exact same arguments like the sort method you saw in the QueryResult object.

Finally, there are also the filter and map methods you can use on Hit objects. Unlike in the QueryResult object, Hit objects only have one variant of filter (Hit.filter) and one variant of map (Hit.map). Both of Hit.filter and Hit.map work on the HSP objects a Hit has.

HSP

HSP (high-scoring pair) represents region(s) in the hit sequence that contains significant alignment(s) to the query sequence. It contains the actual match between your query sequence and a database entry. As this match is determined by the sequence search tool’s algorithms, the HSP object contains the bulk of the statistics computed by the search tool. This also makes the distinction between HSP objects from different search tools more apparent compared to the differences you’ve seen in QueryResult or Hit objects.

Let’s see some examples from our BLAST and BLAT searches. We’ll look at the BLAST HSP first:

from Bio import SearchIO
blast_qresult = SearchIO.read('data/my_blast.xml', 'blast-xml')
blast_hsp = blast_qresult[0][0]    # first hit, first hsp
print(blast_hsp)

      Query: gi|8332116|gb|BE037100.1|BE037100 MP14H09 MP Mesembryanthemum cr...
        Hit: gi|731339628|ref|XM_010682658.1| PREDICTED: Beta vulgaris subsp....
Query range: [86:679] (1)
  Hit range: [80:677] (1)
Quick stats: evalue 1.2e-108; bitscore 405.24
  Fragments: 1 (597 columns)
     Query - TTGGCCATGAAAACTGATCAATTGGCCGTGGCTAATATGATCGATTCCGATATCAATGA~~~TGTAG
             ||||||||||||||||| ||| ||||  |||||||| |||| ||||  ||||| |||||~~~|||||
       Hit - TTGGCCATGAAAACTGAGCAAATGGCGTTGGCTAATTTGATAGATTATGATATGAATGA~~~TGTAG

Just like QueryResult and Hit, invoking print on an HSP shows its general details:

• There are the query and hit IDs and descriptions. We need these to identify our HSP.
• We’ve also got the matching range of the query and hit sequences. The slice notation we’re using here is an indication that the range is displayed using Python’s indexing style (zero-based, half open). The number inside the parenthesis denotes the strand. In this case, both sequences have the plus strand.
• Some quick statistics are available: the e-value and bitscore.
• There is information about the HSP fragments. Ignore this for now; it will be explained later on.
• And finally, we have the query and hit sequence alignment itself.

These details can be accessed on their own using the dot notation, just like in QueryResult and Hit:

blast_hsp.query_range

(86, 679)

blast_hsp.evalue

1.1684e-108

They’re not the only attributes available, though. HSP objects come with a default set of properties that makes it easy to probe their various details. Here are some examples:

blast_hsp.hit_start         # start coordinate of the hit sequence

80

def map_func(hit):
    hit.id = hit.id.split('|')[3]   # renames 'gi|301171322|ref|NR_035857.1|' to 'NR_035857.1'
    return hitblast_hsp.query_span        # how many residues in the query sequence

blast_hsp.aln_span          # how long the alignment is

597

Check out the HSP documentation for a full list of these predefined properties.

Furthermore, each sequence search tool usually computes its own statistics / details for its HSP objects. For example, an XML BLAST search also outputs the number of gaps and identical residues. These attributes can be accessed like so:

blast_hsp.gap_num       # number of gaps

4

blast_hsp.ident_num     # number of identical residues

449

These details are format-specific; they may not be present in other formats. To see which details are available for a given sequence search tool, you should check the format’s documentation in Bio.SearchIO. Alternatively, you may also use .__dict__.keys() for a quick list of what’s available:

blast_hsp.__dict__.keys()

dict_keys(['_items', 'pos_num', 'gap_num', 'bitscore', 'bitscore_raw', 'ident_num', 'evalue'])

Finally, you may have noticed that the query and hit attributes of our HSP are not just regular strings:

blast_hsp.query

SeqRecord(seq=Seq('TTGGCCATGAAAACTGATCAATTGGCCGTGGCTAATATGATCGATTCCGATATC...TAG', DNAAlphabet()), id='gi|8332116|gb|BE037100.1|BE037100', name='aligned query sequence', description="MP14H09 MP Mesembryanthemum crystallinum cDNA 5' similar to cold acclimation protein, mRNA sequence", dbxrefs=[])

blast_hsp.hit

SeqRecord(seq=Seq('TTGGCCATGAAAACTGAGCAAATGGCGTTGGCTAATTTGATAGATTATGATATG...TAG', DNAAlphabet()), id='gi|731339628|ref|XM_010682658.1|', name='aligned hit sequence', description='PREDICTED: Beta vulgaris subsp. vulgaris cold-regulated 413 plasma membrane protein 2 (LOC104895996), mRNA', dbxrefs=[])

They are SeqRecord objects you saw earlier in Section [chapter:SeqRecord]! This means that you can do all sorts of interesting things you can do with SeqRecord objects on HSP.query and/or HSP.hit.

It should not surprise you now that the HSP object has an alignment property which is a MultipleSeqAlignment object:

print(blast_hsp.aln)

DNAAlphabet() alignment with 2 rows and 597 columns
TTGGCCATGAAAACTGATCAATTGGCCGTGGCTAATATGATCGA...TAG gi|8332116|gb|BE037100.1|BE037100
TTGGCCATGAAAACTGAGCAAATGGCGTTGGCTAATTTGATAGA...TAG gi|731339628|ref|XM_010682658.1|

Having probed the BLAST HSP, let’s now take a look at HSPs from our BLAT results for a different kind of HSP. As usual, we’ll begin by invoking print on it:

blat_qresult = SearchIO.read('data/my_blat.psl', 'blat-psl')
blat_hsp = blat_qresult[0][0]       # first hit, first hsp
print(blat_hsp)

      Query: mystery_seq <unknown description>
        Hit: chr19 <unknown description>
Query range: [0:61] (1)
  Hit range: [54204480:54204541] (1)
Quick stats: evalue ?; bitscore ?
  Fragments: 1 (? columns)

Some of the outputs you may have already guessed. We have the query and hit IDs and descriptions and the sequence coordinates. Values for evalue and bitscore is ‘?’ as BLAT HSPs do not have these attributes. But The biggest difference here is that you don’t see any sequence alignments displayed. If you look closer, PSL formats themselves do not have any hit or query sequences, so Bio.SearchIO won’t create any sequence or alignment objects. What happens if you try to access HSP.query, HSP.hit, or HSP.aln? You’ll get the default values for these attributes, which is None:

blat_hsp.hit is None

True

blat_hsp.query is None

True

blat_hsp.aln is None

True

This does not affect other attributes, though. For example, you can still access the length of the query or hit alignment. Despite not displaying any attributes, the PSL format still have this information so Bio.SearchIO can extract them:

blat_hsp.query_span     # length of query match

61

blat_hsp.hit_span       # length of hit match

61

Other format-specific attributes are still present as well:

blat_hsp.score          # PSL score

61

blat_hsp.mismatch_num   # the mismatch column

0

So far so good? Things get more interesting when you look at another ‘variant’ of HSP present in our BLAT results. You might recall that in BLAT searches, sometimes we get our results separated into ‘blocks’. These blocks are essentially alignment fragments that may have some intervening sequence between them.

Let’s take a look at a BLAT HSP that contains multiple blocks to see how Bio.SearchIO deals with this:

blat_hsp2 = blat_qresult[0][1]      # first hit, second hsp
print(blat_hsp2)

      Query: mystery_seq <unknown description>
        Hit: chr19 <unknown description>
Query range: [0:61] (1)
  Hit range: [54233104:54264463] (1)
Quick stats: evalue ?; bitscore ?
  Fragments: ---  --------------  ----------------------  ----------------------
               #            Span             Query range               Hit range
             ---  --------------  ----------------------  ----------------------
               0               ?                  [0:18]     [54233104:54233122]
               1               ?                 [18:61]     [54264420:54264463]

What’s happening here? We still some essential details covered: the IDs and descriptions, the coordinates, and the quick statistics are similar to what you’ve seen before. But the fragments detail is all different. Instead of showing ‘Fragments: 1’, we now have a table with two data rows.

This is how Bio.SearchIO deals with HSPs having multiple fragments. As mentioned before, an HSP alignment may be separated by intervening sequences into fragments. The intervening sequences are not part of the query-hit match, so they should not be considered part of query nor hit sequence. However, they do affect how we deal with sequence coordinates, so we can’t ignore them.

Take a look at the hit coordinate of the HSP above. In the Hit range: field, we see that the coordinate is [54233104:54264463]. But looking at the table rows, we see that not the entire region spanned by this coordinate matches our query. Specifically, the intervening region spans from 54233122 to 54264420.

Why then, is the query coordinates seem to be contiguous, you ask? This is perfectly fine. In this case it means that the query match is contiguous (no intervening regions), while the hit match is not.

All these attributes are accessible from the HSP directly, by the way:

blat_hsp2.hit_range         # hit start and end coordinates of the entire HSP

(54233104, 54264463)

blat_hsp2.hit_range_all     # hit start and end coordinates of each fragment

[(54233104, 54233122), (54264420, 54264463)]

blat_hsp2.hit_span          # hit span of the entire HSP

31359

blat_hsp2.hit_span_all      # hit span of each fragment

[18, 43]

blat_hsp2.hit_inter_ranges  # start and end coordinates of intervening regions in the hit sequence

[(54233122, 54264420)]

blat_hsp2.hit_inter_spans   # span of intervening regions in the hit sequence

[31298]

Most of these attributes are not readily available from the PSL file we have, but Bio.SearchIO calculates them for you on the fly when you parse the PSL file. All it needs are the start and end coordinates of each fragment.

What about the query, hit, and aln attributes? If the HSP has multiple fragments, you won’t be able to use these attributes as they only fetch single SeqRecord or MultipleSeqAlignment objects. However, you can use their *_all counterparts: query_all, hit_all, and aln_all. These properties will return a list containing SeqRecord or MultipleSeqAlignment objects from each of the HSP fragment. There are other attributes that behave similarly, i.e. they only work for HSPs with one fragment. Check out the HSP documentation for a full list.

Finally, to check whether you have multiple fragments or not, you can use the is_fragmented property like so:

blat_hsp2.is_fragmented     # BLAT HSP with 2 fragments

True

blat_hsp.is_fragmented      # BLAT HSP from earlier, with one fragment

False

Before we move on, you should also know that we can use the slice notation on HSP objects, just like QueryResult or Hit objects. When you use this notation, you’ll get an HSPFragment object in return, the last component of the object model.

HSPFragment

HSPFragment represents a single, contiguous match between the query and hit sequences. You could consider it the core of the object model and search result, since it is the presence of these fragments that determine whether your search have results or not.

In most cases, you don’t have to deal with HSPFragment objects directly since not that many sequence search tools fragment their HSPs. When you do have to deal with them, what you should remember is that HSPFragment objects were written with to be as compact as possible. In most cases, they only contain attributes directly related to sequences: strands, reading frames, alphabets, coordinates, the sequences themselves, and their IDs and descriptions.

These attributes are readily shown when you invoke print on an HSPFragment. Here’s an example, taken from our BLAST search:

from Bio import SearchIO
blast_qresult = SearchIO.read('data/my_blast.xml', 'blast-xml')
blast_frag = blast_qresult[0][0][0]    # first hit, first hsp, first fragment
print(blast_frag)

      Query: gi|8332116|gb|BE037100.1|BE037100 MP14H09 MP Mesembryanthemum cr...
        Hit: gi|731339628|ref|XM_010682658.1| PREDICTED: Beta vulgaris subsp....
Query range: [86:679] (1)
  Hit range: [80:677] (1)
  Fragments: 1 (597 columns)
     Query - TTGGCCATGAAAACTGATCAATTGGCCGTGGCTAATATGATCGATTCCGATATCAATGA~~~TGTAG
             ||||||||||||||||| ||| ||||  |||||||| |||| ||||  ||||| |||||~~~|||||
       Hit - TTGGCCATGAAAACTGAGCAAATGGCGTTGGCTAATTTGATAGATTATGATATGAATGA~~~TGTAG

At this level, the BLAT fragment looks quite similar to the BLAST fragment, save for the query and hit sequences which are not present:

blat_qresult = SearchIO.read('data/my_blat.psl', 'blat-psl')
blat_frag = blat_qresult[0][0][0]    # first hit, first hsp, first fragment
print(blat_frag)

      Query: mystery_seq <unknown description>
        Hit: chr19 <unknown description>
Query range: [0:61] (1)
  Hit range: [54204480:54204541] (1)
  Fragments: 1 (? columns)

In all cases, these attributes are accessible using our favorite dot notation. Some examples:

blast_frag.query_start      # query start coordinate

86

blast_frag.hit_strand       # hit sequence strand

1

blast_frag.hit              # hit sequence, as a SeqRecord object

SeqRecord(seq=Seq('TTGGCCATGAAAACTGAGCAAATGGCGTTGGCTAATTTGATAGATTATGATATG...TAG', DNAAlphabet()), id='gi|731339628|ref|XM_010682658.1|', name='aligned hit sequence', description='PREDICTED: Beta vulgaris subsp. vulgaris cold-regulated 413 plasma membrane protein 2 (LOC104895996), mRNA', dbxrefs=[])

A note about standards and conventions

Before we move on to the main functions, there is something you ought to know about the standards Bio.SearchIO uses. If you’ve worked with multiple sequence search tools, you might have had to deal with the many different ways each program deals with things like sequence coordinates. It might not have been a pleasant experience as these search tools usually have their own standards. For example, one tools might use one-based coordinates, while the other uses zero-based coordinates. Or, one program might reverse the start and end coordinates if the strand is minus, while others don’t. In short, these often creates unnecessary mess must be dealt with.

We realize this problem ourselves and we intend to address it in Bio.SearchIO. After all, one of the goals of Bio.SearchIO is to create a common, easy to use interface to deal with various search output files. This means creating standards that extend beyond the object model you just saw.

Now, you might complain, “Not another standard!”. Well, eventually we have to choose one convention or the other, so this is necessary. Plus, we’re not creating something entirely new here; just adopting a standard we think is best for a Python programmer (it is Biopython, after all).

There are three implicit standards that you can expect when working with Bio.SearchIO:

• The first one pertains to sequence coordinates. In Bio.SearchIO, all sequence coordinates follows Python’s coordinate style: zero-based and half open. For example, if in a BLAST XML output file the start and end coordinates of an HSP are 10 and 28, they would become 9 and 28 in Bio.SearchIO. The start coordinate becomes 9 because Python indices start from zero, while the end coordinate remains 28 as Python slices omit the last item in an interval.
• The second is on sequence coordinate orders. In Bio.SearchIO, start coordinates are always less than or equal to end coordinates. This isn’t always the case with all sequence search tools, as some of them have larger start coordinates when the sequence strand is minus.
• The last one is on strand and reading frame values. For strands, there are only four valid choices: 1 (plus strand), -1 (minus strand), 0 (protein sequences), and None (no strand). For reading frames, the valid choices are integers from -3 to 3 and None.

Note that these standards only exist in Bio.SearchIO objects. If you write Bio.SearchIO objects into an output format, Bio.SearchIO will use the format’s standard for the output. It does not force its standard over to your output file.

Reading search output files

There are two functions you can use for reading search output files into Bio.SearchIO objects: read and parse. They’re essentially similar to read and parse functions in other submodules like Bio.SeqIO or Bio.AlignIO. In both cases, you need to supply the search output file name and the file format name, both as Python strings. You can check the documentation for a list of format names Bio.SearchIO recognizes.

Bio.SearchIO.read is used for reading search output files with only one query and returns a QueryResult object. You’ve seen read used in our previous examples. What you haven’t seen is that read may also accept additional keyword arguments, depending on the file format.

Here are some examples. In the first one, we use read just like previously to read a BLAST tabular output file. In the second one, we use a keyword argument to modify so it parses the BLAST tabular variant with comments in it:

from Bio import SearchIO
qresult = SearchIO.read('data/tab_2226_tblastn_003.txt', 'blast-tab')
qresult

QueryResult(id='gi|16080617|ref|NP_391444.1|', 3 hits)

qresult2 = SearchIO.read('data/tab_2226_tblastn_007.txt', 'blast-tab', comments=True)
qresult2

QueryResult(id='gi|16080617|ref|NP_391444.1|', 3 hits)

These keyword arguments differs among file formats. Check the format documentation to see if it has keyword arguments that modifies its parser’s behavior.

As for the Bio.SearchIO.parse, it is used for reading search output files with any number of queries. The function returns a generator object that yields a QueryResult object in each iteration. Like Bio.SearchIO.read, it also accepts format-specific keyword arguments:

from Bio import SearchIO
qresults = SearchIO.parse('data/tab_2226_tblastn_001.txt', 'blast-tab')
for qresult in qresults:
    print(qresult.id)

gi|16080617|ref|NP_391444.1|
gi|11464971:4-101

qresults2 = SearchIO.parse('data/tab_2226_tblastn_005.txt', 'blast-tab', comments=True)
for qresult in qresults2:
    print(qresult.id)

random_s00
gi|16080617|ref|NP_391444.1|
gi|11464971:4-101

Dealing with large search output files with indexing

Sometimes, you’re handed a search output file containing hundreds or thousands of queries that you need to parse. You can of course use Bio.SearchIO.parse for this file, but that would be grossly inefficient if you need to access only a few of the queries. This is because parse will parse all queries it sees before it fetches your query of interest.

In this case, the ideal choice would be to index the file using Bio.SearchIO.index or Bio.SearchIO.index_db. If the names sound familiar, it’s because you’ve seen them before in Section [sec:SeqIO-index]. These functions also behave similarly to their Bio.SeqIO counterparts, with the addition of format-specific keyword arguments.

Here are some examples. You can use index with just the filename and format name:

from Bio import SearchIO
idx = SearchIO.index('data/tab_2226_tblastn_001.txt', 'blast-tab')
sorted(idx.keys())

['gi|11464971:4-101', 'gi|16080617|ref|NP_391444.1|']

idx['gi|16080617|ref|NP_391444.1|']

QueryResult(id='gi|16080617|ref|NP_391444.1|', 3 hits)

idx.close()

Or also with the format-specific keyword argument:

idx = SearchIO.index('data/tab_2226_tblastn_005.txt', 'blast-tab', comments=True)
sorted(idx.keys())

['gi|11464971:4-101', 'gi|16080617|ref|NP_391444.1|', 'random_s00']

idx['gi|16080617|ref|NP_391444.1|']

QueryResult(id='gi|16080617|ref|NP_391444.1|', 3 hits)

idx.close()

Or with the key_function argument, as in Bio.SeqIO:

key_function = lambda id: id.upper()    # capitalizes the keys
idx = SearchIO.index('data/tab_2226_tblastn_001.txt', 'blast-tab', key_function=key_function)
sorted(idx.keys())

['GI|11464971:4-101', 'GI|16080617|REF|NP_391444.1|']

idx['GI|16080617|REF|NP_391444.1|']

QueryResult(id='gi|16080617|ref|NP_391444.1|', 3 hits)

idx.close()

Bio.SearchIO.index_db works like as index, only it writes the query offsets into an SQLite database file.

Writing and converting search output files

It is occasionally useful to be able to manipulate search results from an output file and write it again to a new file. Bio.SearchIO provides a write function that lets you do exactly this. It takes as its arguments an iterable returning QueryResult objects, the output filename to write to, the format name to write to, and optionally some format-specific keyword arguments. It returns a four-item tuple, which denotes the number or QueryResult, Hit, HSP, and HSPFragment objects that were written.

from Bio import SearchIO
qresults = SearchIO.parse('data/mirna.xml', 'blast-xml')     # read XML file
SearchIO.write(qresults, 'results.tab', 'blast-tab')    # write to tabular file

(3, 239, 277, 277)

You should note different file formats require different attributes of the QueryResult, Hit, HSP and HSPFragment objects. If these attributes are not present, writing won’t work. In other words, you can’t always write to the output format that you want. For example, if you read a BLAST XML file, you wouldn’t be able to write the results to a PSL file as PSL files require attributes not calculated by BLAST (e.g. the number of repeat matches). You can always set these attributes manually, if you really want to write to PSL, though.

Like read, parse, index, and index_db, write also accepts format-specific keyword arguments. Check out the documentation for a complete list of formats Bio.SearchIO can write to and their arguments.

Finally, Bio.SearchIO also provides a convert function, which is simply a shortcut for Bio.SearchIO.parse and Bio.SearchIO.write. Using the convert function, our example above would be:

from Bio import SearchIO
SearchIO.convert('data/mirna.xml', 'blast-xml', 'results.tab', 'blast-tab')

(3, 239, 277, 277)